Biotechnology & nanotechnology
Madjid Momeni-Moghaddam; Roya Gholami
Volume 26, Issue 5 , November and December 2019, , Pages 651-656
Abstract
Abstract:Aims: Collagen synthesis can help to heal wounds, especially Large wounds. Any treatments that can stimulate collagen synthesis in fibroblasts can accelerate wound repair, so it has clinical value. Stem cells secrete some macromolecules and signals that can have different effects in other cells.Materials ...
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Abstract:Aims: Collagen synthesis can help to heal wounds, especially Large wounds. Any treatments that can stimulate collagen synthesis in fibroblasts can accelerate wound repair, so it has clinical value. Stem cells secrete some macromolecules and signals that can have different effects in other cells.Materials and Methods: In this study, human mesenchymal stem cells were cultured and their medium supernatant was harvested as a medium containing the signal. After determining concentration, they were exposed to mouse fibroblasts. The cell viability was examined and then in the molecular section, by designing specific primers, three The collagen gene was evaluated by Real-Time PCR.Results: The results indicated a growth-promoting effect using the amount of 20 μg /ml of stem cells conditioned media in the culture medium of fibroblast cells. It was also found in the molecular section, that this concentration could increase the expression of all three selected collagen genes.Discussion and conclusion: The results of this study fully confirmed the initial goals, indicating the high power of mesenchymal stem cells in the induction of collagen synthesis, and thus could be a very good candidate for clinical studies.
Fatemeh Nahidi; Roya Gholami
Volume 20, Issue 4 , January and February 2014, , Pages 479-486
Abstract
Background& Purpose: Preeclampsia is a significant threat to public health and the leading cause of maternal and fetal death and disability worldwide. Its incidence is between 5 to 12 percent in Iran, which has many causes.Husband’s occupation is one of these factors that has not been considered ...
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Background& Purpose: Preeclampsia is a significant threat to public health and the leading cause of maternal and fetal death and disability worldwide. Its incidence is between 5 to 12 percent in Iran, which has many causes.Husband’s occupation is one of these factors that has not been considered till now. This study was performed to determine the effect of husband’s occupation on preeclampsia in pregnant women in Tehran.
Materials & Methods: This is a retrospective study with a total case of 195 pregnant women (65 women with preeclampsia and 130 controls) admitted to selected hospitals in Tehran, 2011. Among the hospitals with higher number of deliveriesin the north, south, east, west and central of Tehran, 4 reference hospitals wereselected based onobjectiveand non-randomized methodand given referrals for prenatal care and delivery; every hospital was given a quota. Data collecting tools were questionnaire and checklist that were filled by interview with qualified women and their cases. Women were divided into worker, employee, unemployed and self employed groups. Confounding factors were controlledthrough randomizing. We used independent t test for quantitative variables and chi-square test for relationships between categorical variables. Odds ratio was used to show the statistics relationship between some variables and preeclampsia.All data analysiswas performed bySPSSversion 18.
Results: The results of this study showed that there is a statistically significant association between husband’s occupationand preeclampsia (P=0.01). In preeclamptic group, the worker husbands and in healthy group the self employed husbands were the most.
Conclusion: It seems that husband’s occupation is related to preeclampsia and the incidence of preeclampsia in worker’s family is more than families that are employee and self-employed.